Synonyms:
A-152E5.1, ABCD-1, DC/B-CK, MDC, CC chemokine STCP-1, macrophage-derived chemokine, small inducible cytokine A22, small inducible cytokine subfamily A (Cys-Cys), member 22, stimulated T cell chemotactic protein 1
Description:
Recombinant Human MDC/CCL22 is a single, non-glycosylated polypeptide chain containing 69 amino acids. Background: CCL22 is a protein that in humans is encoded by the CCL22 gene, which locates on the Chr. 16. The protein is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).
Molecular Weight:
~8.1 kDa
Formulation:
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 500 mM NaCl.
Purity:
>97 % by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.
Amino Acid Sequence:
GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilut
Storage/Stability:
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the r
