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Recombinant Human LONG®R3 IGF-I (Liquid)

Molecule:

IGF-I

Synonyms:

Insulin-like growth factor I, IGF-I, Mechano growth factor, Somatomedin-C

Species:

Human

Cat. No.:

CRH061 pdf (datasheet)

Quantity/Size:

5 ml

Price:

Please Inquire

Description:

LONG® R3 IGF-I is an analog of IGF-1 that was developed for use in mammalian serum free cell culture to promote cell growth, survival, and recombinant protein expression. When supplemented in serum-free medium, it promotes cell proliferation, increases cell survival, and enhances productivity through better proliferative and anti-apoptotic signaling. It has an increased half-life in vitro and in vivo and a low affinity for IGF binding proteins (IGFBP) that make it ideal for both research and large-scale culturing.

The protein was created by substituting an arginine (R) for glutamic acid (E) at amino acid 3 and extending the N-terminal end of the peptide by 13 amino acids (MFPAMPLSSLFVN). All cell types that have a growth response to insulin in cell culture have the potential to respond to LONG® R3 IGF-I.

Recombinant Human LONG® R3 IGF-I is a single, non-glycosylated polypeptide chain containing 83 amino acids.

Concentration:

1.0 mg/ml by RP-HPLC, lot specific

Uniprot Name:

Insulin-like growth factor I

Uniprot ID:

P05019

Data PDF:

CRH061

Source:

E. coli

Molecular Weight:

~9.1 kDa as determined by mass spectrometry (9108-9112 Da)

Formulation:

0.1 M acetic acid

Purity:

>95% single band by SDS-PAGE with the same electrophoretic mobility as the reference standard.

Biological Activity:

ED50 < 10 ng/ml, determined by the stimulation of protein synthesis in L6 myoblasts.

Endotoxin Level:

<0.1 EU/μg as determined by LAL assay

Amino Acid Sequence:

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Storage/Stability:

Store at 2-8°C

Application notes:

Cell culture supplement for use in serum-free media to enhance survival and proliferation of mammalian cells.