Recombinant Human Interleukin-18 GMP

Molecule:

IL-18

Synonyms:

Interleukin-18, IL-18, Interferon gamma-inducing factor, IGIF, Interleukin-1 gamma

Species:

Human

Cat. No.:

CRI166B-GMP pdf (datasheet)

Quantity/Size:

100 μg

Price:

$850.00 BUY

Description:

Human Interleukin-18 is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation. IL-18 binds to the widely expressedIL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs.

The manufacturing and testing of Recombinant Human Interleukin-18 GMP complies with ICH Q7 guidelines.

Entrez Symbol:

IL18

Uniprot Name:

Interleukin-18

Uniprot ID:

Q14116

Data PDF:

CRI166-GMP

Source:

E. coli

Molecular Weight:

18.2 kDa (157 aa) monomer

Formulation:

Lyophilized from sterile filtered PBS, 0.05% Tween-20, pH 7.0

Purity:

> 95% by SDS-PAGE
> 98% by HPLC

Biological Activity:

ED50 = 1.5-15 ng/ml, determined by the ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells

Specific Activity:

> 3.0 x 106 Units/mg, calibrated against the rhIL-18 WHO reference standard (NIBSC code: 03/200) 

Endotoxin Level:

< 0.01 EU/μg as determined by LAL method.

Amino Acid Sequence:

YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIIS MYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDII FFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIM FTVQNED

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.