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Recombinant Human Interferon alpha-4 (Fc Tag)

Synonyms:

Interferon alpha-4, IFN-alpha-4, Interferon alpha-4B, Interferon alpha-76, Interferon alpha-M1

Species:

Human

Cat. No.:

CRH454D-Fc pdf (datasheet)

Quantity/Size:

100 µg

Price:

$360.00 BUY

Description:

Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 22 and 41 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

Uniprot Name:

Interferon alpha-4

Uniprot ID:

P05014

Data PDF:

CRH454-Fc

Genbank Accession No:

NP_066546.1

Source:

HEK293 cells

Molecular Weight:

The rhFc/IFN-α4a is a disulfide-linked homodimer. The reduced monomer consists of 403 aa and has a predicted MW of 46 kDa. Due to glycosylation, the apparent MW of rh Fc/IFN-α4a monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.

Tag:

Fc

Formulation:

Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Purity:

> 97 % as determined by SDS-PAGE

Biological Activity:

Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV).
The EC50 for this effect is 0.2-1 ng/mL.

Endotoxin Level:

< 1.0 EU per μg protein as determined by the LAL method

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Stable for up to 1 year from date of receipt at -20°C to -80°C After reconstitution, store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.