Synonyms:
interleukin-33, IL-33, IL-1F11, DVS27-related protein, interleukin-1 family member 11, nuclear factor from high endothelial venules
Description:
Recombinant Human IL-33
Molecular Weight:
Monomer, 18.1 kDa (160 aa)
Physical State:
Lyophilized
Formulation:
Sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Purity:
> 95.0% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 500 pg/ml, determined by a cell proliferation assay using mouse 10S cells.
Specific Activity:
≤2.0 × 10e6 U/mg
Endotoxin Level:
<1 EU/μg by kinetic LAL method.
Amino Acid Sequence:
MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store at -20°C to -80° for up to 1 year.