Description:
Recombinant Human IL-31
Uniprot Name:
Interleukin-31
Molecular Weight:
15.8 kDa (141 aa) monomer
Formulation:
Lyophilized from a sterile filtered solution containing 20 mM sodium phosphate, 50 mM NaCl, pH 7.5
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
≤ 25 ng/ml, demonstrates STAT3 activation of U-87 MG cells.
Endotoxin Level:
≤ 1 EU/μg, determined by kinetic LAL analysis.
Amino Acid Sequence:
SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for reconstitution. A small amount of precipitate may be seen.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
