Recombinant Human IL-3, GMP Grade

Synonyms:

Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor

Species:

Human

Cat. No.:

CRH311A-GMP pdf (datasheet)

Quantity/Size:

50 μg

Price:

$1,840.00 BUY

Description:

Interleukin 3, produced by activated T cells and mast cells, induces the differentiation of hematopoietic stem cells into myeloid precursor cells, such as erythrocyte, megakaryocyte, granulocyte, monocyte, and dendritic cells. IL-3 also functions in the nervous system and during B-1 cell regulation of chronic inflammatory diseases.

This product is produced from E. coli and is manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. This product is manufactured, tested, and released in an ISO 9001:2015 certified facility and follows cGMP practices. USP chapter <1043> for ancillary materials considered in the manufacture of this product.

Entrez Symbol:

IL3

Uniprot Name:

Interleukin-3

Uniprot ID:

P08700

Data PDF:

CRH311-GMP

Source:

E. coli

Molecular Weight:

15.2 kDa (134 aa) monomer

Formulation:

Lyophilized (1mg/ml) sterile-filtered 10 mM sodium phosphate, 150 mM NaCl, pH 7.5.

Purity:

> 98% by reducing and non-reducing SDS-PAGE

Biological Activity:

 ED50 < 2 ng/ml, determined by dose-dependent proliferation of human TF-1 cells.  

Specific Activity:

~4.2 x 103 IU/µg, which is calibrated against the recombinant Human IL-3 WHO International Standard (NIBSC code 91/510).

Endotoxin Level:

< 0.05 EU/µg using USP <85> / EP 2.6.14

Amino Acid Sequence:

MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF

Reconstitution:

Allow the vial and sterile water/Water for Injection to equilibrate to room temperature. Draw up desired volume for reconstitution with sterile syringe. Aseptically puncture through rubber stopper with sterile needle, inject the buffer to achieve the desired concentration (0.1-0.5 mg/mL). Swirl the vial gently, do not vortex. Allow protein to rehydrate for 10-15 minutes at room temperature with occasional gentle mixing.

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. 

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.