Synonyms:
IL-21, CVID11, Za11
Description:
Interleukin 21 (IL-21) is a member of the common-gamma chain family of cytokines with immunoregulatory activity. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer (NK) cells, B cells and cytotoxic T cells. IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors. The IL-21R activates the JAK/STAT signaling pathway and is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 is a switch factor regulating IgG1 and IgG3 antibody production. IL-21 also cooperates with IL-4 for the production of multiple antibody classes in B cells.
Uniprot Name:
Interleukin-21
Molecular Weight:
15.4 kDa (132 aa)
Formulation:
Lyophilized from sterile-filtered 10 mM sodium phosphate, pH 7.5.
Purity:
>95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 < 5 ng/ml, determined by dose-dependent proliferation of B9 cells
Specific Activity:
> 2.0 × 105 U/mg
Endotoxin Level:
≤1 EU/µg of protein by USP <85>/ EP 2.6.14
Amino Acid Sequence:
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year.
