IL-2

Interleukin-2, IL-2, T-cell growth factor, TCGF

Human

CRI254B pdf (datasheet)

50 μg

$350.00 BUY

Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL-2 may be of use as an anti-tumor agent in cancer therapy. The Recombinant Human IL-2 contains 133 amino acids and a 16 aa His-tag for a total of 149 a.a.

~ 0.50 mg/ml prior to lyophilization, lot specific

Interleukin-2

P60568

CRI254

Barley

Predicted MW 17.5 kDa (133 aa + 16x His tag = total 149 aa)
Apparent MW 22 kDa in SDS-PAGE, due to glycosylation

Lyophilized from sterile-filtered 2X PBS, pH 7.2.

>95% by SDS-PAGE

ED₅₀ < 1.5 ng/ml, determined by the dose-dependent effect of human IL-2 on proliferation of CTLL-2 cells.

> 1.0 x 10⁶ U/mg, lot specific

< 0.05 EU/µg as measured by turbidimetric kinetic assay.

Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed.

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C to -80°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.