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Recombinant Human IL-2 (C126S), GMP Grade

Synonyms:

Interleukin-2, IL-2, T-cell Growth Factor, TCGF, Aldesleukin

Species:

Human

Cat. No.:

CRI101A-GMP pdf (datasheet)

Quantity/Size:

50 μg

Price:

$745.00 BUY

Description:

Interleukin-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. GMP Grade amino acid sequence including C126S is identical to aldesleukin.

This product is produced from E. coli and is manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. This product is manufactured, tested, and released in an ISO 9001:2015 certified facility and follows cGMP practices. USP chapter <1043> for ancillary materials considered in the manufacture of this product.

Entrez Symbol:

IL2

Uniprot Name:

Interleukin-2

Uniprot ID:

P60568

Data PDF:

CRI101-GMP

Source:

E. coli

Molecular Weight:

15.5 kDa (134 aa) monomer

Formulation:

Lyophilized (1 mg/ml) sterile-filtered solution containing 0.1% Trifluoroacetic Acid (TFA)

Purity:

> 97% by reducing and non-reducing SDS-PAGE

Biological Activity:

 ED50 < 3 ng/ml, determined by dose-dependent proliferation of mouse CTLL-2 cells.  

Specific Activity:

~1.8 x 104 IU/µg, which is calibrated against the recombinant Human IL-2 WHO International Standard (NIBSC code 86/500).

Endotoxin Level:

<0.01 EU/µg using USP <85>/ EP 2.6.14

Amino Acid Sequence:

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFSQSII STLT

Reconstitution:

Allow the vial and sterile water/Water for Injection to equilibrate to room temperature. Draw up desired volume for reconstitution with sterile syringe. Aseptically puncture through rubber stopper with sterile needle, inject the buffer to achieve the desired concentration (0.1-0.5 mg/mL). Swirl the vial gently, do not vortex. Allow protein to rehydrate for 10-15 minutes at room temperature with occasional gentle mixing.

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. 

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.