Recombinant Human IL-1RA/IL-1RN

Molecule:

IL-1RA

Synonyms:

Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IL-1RAP, IL-1 inhibitor

Species:

Human

Cat. No.:

CRI134B pdf (datasheet)

Quantity/Size:

100 μg

Price:

$275.00 BUY

Description:

Interleukin-1 receptor antagonist (IL-1ra) is a member of the IL-1 family. Endogenous IL-1ra is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-ra. The regulated expression of IL-1ra in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1ra is markedly enhanced by IL-1, TNF-alpha, or PDGF.

Entrez Symbol:

IL1RN

Uniprot Name:

Interleukin-1 receptor antagonist protein

Uniprot ID:

P18510

Data PDF:

CRI134.

Source:

E. coli

Molecular Weight:

17.1 kDa (152 aa) monomer

Formulation:

Lyophilized from a sterile filtered solution in PBS, pH 7.4

Purity:

>96% by HPLC and SDS-PAGE

Biological Activity:

ED50 < 40 ng/ml, determined by inhibiting IL-1α-dependent proliferation of murine D10S cells in the presence of 50 pg/ml rHuIL-1α.

Specific Activity:

> 2.5 × 10e4 IU/mg

Endotoxin Level:

< 1 EU/µg of rHuIL-1RA as determined by LAL method.

Amino Acid Sequence:

RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE

Storage/Stability:

Store at -20°C to -80°C for up to 1 year.