Recombinant Human IL-17A

Molecule:

IL-17A

Synonyms:

CTLA-8, IL17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8

Species:

Human

Cat. No.:

CRH065A pdf (datasheet)

Quantity/Size:

5 µg

Price:

$135.00 BUY

Description:

Human Interleukin 17A (IL-17A) is a pro-inflammatory cytokine member of a six-species family of proteins (IL-17A-17F). IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most importantly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis.

Recombinant Human Interleukin-17A is a disulfide-linked homodimer of two 137 amino acid polypeptide chains.

Entrez Symbol:

IL17A

Uniprot ID:

Q16552

Data PDF:

CRH065

Source:

E. coli

Molecular Weight:

15.7 kDa (monomer)/ 31.3 kDa (dimer)

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by IL-6 production by mouse NIH/3T3 cells, is < 10 ng/ml.

Specific Activity:

> 1 x 105 units/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.