Synonyms:
AGIF (Adipogenesis Inhibitory Factor), oprelvekin, IL-11
Description:
Recombinant Human IL-11
Molecular Weight:
19.3 kDa (179 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from a 0.2μm filtered aqueous solution containing 0.1% Trifluoroacetic acid (TFA).
Purity:
>95%, by reducing and non-reducing SDS-PAGE.
Biological Activity:
ED50 ≤ 10 ng/ml, determined by a cell proliferation assay using TF-1 cells. ED50 ≤ 2.5 ng/ml, determined by a cell proliferation assay using T11 cells.
Specific Activity:
> 1.0 × 10e6 IU/mg, by proliferation assay using TF-1 cells. > 4.0 × 10e6 IU/mg, by proliferation assay using T11 cells.
Endotoxin Level:
< 1EU/μg, by kinetic LAL.
Amino Acid Sequence:
MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store at -20°C to -80°C for up to 1 year.
