Synonyms:
CSIF, TGIF, GVHDS, IL-10, IL10A
Description:
Recombinant Human IL-10
Uniprot Name:
Interleukin-10
Molecular Weight:
Noncovalent homodimer, 18.8 kDa (161 aa)
Formulation:
Sterile filtered solution of 10 mM sodium phosphate, pH 7.5
Purity:
≥95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤5 ng/ml, determined by proliferation of mouse MC/9 cells
Specific Activity:
≥2.0 x 105 units/mg, calibrated against recombinant human IL-10 WHO International Standard (NIBSC code: 93/722)
Endotoxin Level:
≤1 EU/µg, by kinetic LAL
Amino Acid Sequence:
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Upon receipt, store as supplied at -20°C to -80°C for up to one year. Upon reconstitution store at 2-8°C for up to one month. For long term storage reconstitute in working aliquots containing 0.1% BSA and store at -80°C. Avoid repeated freeze-thaw cycles.
