Recombinant Human IL-1 beta, GMP Grade

Synonyms:

Interleukin-1 beta, IL-1 beta, Catabolin

Species:

Human

Cat. No.:

CRI135A-GMP pdf (datasheet)

Quantity/Size:

5 μg

Price:

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Description:

Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties; The mature human IL1β shares 96% amino acid sequence identity with rhesus and 67% - 78% with canine, mouse, and rat IL-1β.
Manufacturing and testing of this product complies with ICH Q7 guidelines.

Entrez Symbol:

IL1B

Uniprot Name:

Interleukin-1 beta

Uniprot ID:

P01584

Data PDF:

CRI135-GMP

Source:

E. coli

Molecular Weight:

~17.4 kDa (153 aa) monomer

Formulation:

Lyophilized from sterile-filtered PBS, 3% Trehalose, pH 5.0

Purity:

> 98.0% by HPLC and SDS-PAGE

Biological Activity:

ED50 < 1.0 pg/ml, determined by dose-dependent cell proliferation assay using murine D10S cells.

Specific Activity:

> 1.0 × 10e9 U/mg

Endotoxin Level:

< 0.01 EU/μg protein as determined by LAL method

Amino Acid Sequence:

APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.