Recombinant Human IL-1 alpha, GMP Grade

Synonyms:

Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

Species:

Human

Cat. No.:

CRI136B-GMP pdf (datasheet)

Quantity/Size:

100 μg

Price:

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Description:

Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar identical biological properties. Among various species, the amino acid sequence of mature IL-1α is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines.
Manufacturing and testing of this product complies with ICH Q7 guidelines.

Entrez Symbol:

IL1A

Uniprot Name:

Interleukin-1 alpha

Uniprot ID:

P01583

Data PDF:

CRI136-GMP

Source:

E. coli

Molecular Weight:

~18.0 kDa (159 aa) monomer

Formulation:

Lyophilized from sterile-filtered 25 mM Tris-HCl, 3 mM 2-Me, 5% Trehalose, pH 8.0

Purity:

> 98.0% by HPLC and SDS-PAGE

Biological Activity:

ED50 < 1.0 pg/ml, determined by dose-dependent cell proliferation assay using murine D10S cells.

Specific Activity:

> 1.0 × 10e9 U/mg

Endotoxin Level:

< 0.01 EU/μg protein as determined by LAL method

Amino Acid Sequence:

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.