Synonyms:
IGF-BP1, Insulin-like growth factor-binding protein 1, Placental protein 12, PP12
Description:
IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, deciduas, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds to both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, where as nonphosphorylated IGF-BP1 is stimulatory.
Molecular Weight:
25.4 kDa (235 aa)
Formulation:
Lyophilized from PBS
Purity:
> 98% by SDS-PAGE and HPLC analysis
Biological Activity:
ED50 is < 0.5 μg/ml , determined by its ability to inhibit IGF-I induced proliferation of MCF -7, in the presence of 6 ng/ml of human IGF-I.
Endotoxin Level:
< 1 EU/µg
Amino Acid Sequence:
MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN
Storage/Stability:
Stable for 1 year at -20°C to -80°C.
