Synonyms:
Somatomedin A, GRDF
Description:
Recombinant Human IGF-II
Uniprot Name:
Insulin-like growth factor II
Molecular Weight:
Monomer, 7.5 kDa (67 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
≥95% by reducing and non-reducing SDS-PAGE.
Biological Activity:
The ED50, calculated by the dose-dependent proliferation of FDC-P1 cells, is ≤15 ng/mL
Specific Activity:
≥ 6.7 X 104 U/mg
Endotoxin Level:
≤ 0.1 EU/µg by kinetic LAL analysis
Amino Acid Sequence:
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Reconstitution:
Centrifuge vial before opening. Reconstitute with sterile water at a concentration of 0.1 mg/mL, gently pipet up and down the sides of the vial to ensure full recovery of the protein into solution.
Storage/Stability:
Upon receipt, store at -20°C to -80°C for up to one year. Upon reconstitution as directed, the preparation is stable for up to 1 month at 2-8°C, 3 months at -20°C to -80°C. Long term storage reconstitute in working aliquots containing 0.1% BSA and store at -80°C. Avoid repeated freeze-thaw cycles
