Recombinant Human IGF-I LR3, Endotoxin Free

Synonyms:

Insulin-like growth factor I

Cat. No.:

CRI246B pdf (datasheet)

Quantity/Size:

100 µg

Price:

$300.00 BUY

Description:

Human LR3 insulin-like Growth Factor-I (LR3IGF-I) is an analog of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus. Human LR3IGF-I is more potent than native IGF-I in vitro and in vivo. This increased potency is due to reduced binding of LR3IGF-I to most of the IGF binding proteins which modify the biological actions of IGF-I.

Uniprot Name:

Insulin-like growth factor 1

Uniprot ID:

P05019

Source:

Barley

Molecular Weight:

9.1 kDa, predicted (83 aa + 16x His tag) migrates at 12.4 kDa on SDS-PAGE

Formulation:

PBS, pH 7.2

Purity:

>95% by SDS-PAGE analysis

Biological Activity:

ED50 < 2 ng/ml, determined by dose-dependent proliferation of FDC-P1 cells.

Specific Activity:

> 5.0 x 105 U/mg

Endotoxin Level:

< 0.1 EU/mgby kinetic LAL analysis No pyrogenic or pro-inflammatory contaminants by monocyte activation test (MAT)

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX . Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, store at 2-8°C for up to 1 month or prepare working aliquots and store at -20°C to -80°C for up to 3 months. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.