Recombinant Human IGF-I, GMP Grade

Synonyms:

Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C

Species:

Human

Cat. No.:

CRI510B-GMP pdf (datasheet)

Quantity/Size:

100 μg

Price:

Please Inquire

Description:

Insulin-like growth factors (IGFs) belonging to the insulin gene family, are mitogenic polypeptides that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar in structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.
Manufacturing and testing of this product complies with ICH Q7 guidelines.

Entrez Symbol:

IGF1

Uniprot Name:

Insulin-like growth factor I

Uniprot ID:

P05019

Data PDF:

CRI510-GMP

Source:

E. coli

Molecular Weight:

~7.6 kDa (70 aa) monomer

Formulation:

Lyophilized from sterile-filtered PBS, pH 7.0

Purity:

> 98.0% by HPLC and SDS-PAGE

Biological Activity:

ED50 < 2.0 ng/ml, determined by dose-dependent cell proliferation assay using serum free human MCF-7 cells.

Specific Activity:

> 5.0 × 10e5 U/mg

Endotoxin Level:

< 0.01 EU/μg protein as determined by LAL method

Amino Acid Sequence:

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.