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Recombinant Human Insulin-like Growth Factor I

Molecule:

IGF-I

Synonyms:

IGF-I, Somatomedin-C, Mechano growth factor, MGF

Species:

Human

Cat. No.:

CRI500C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$310.00 BUY

Description:

Insulin-like growth factor 1 (IGF-I) is a member of the IGF family of mitogenic peptide growth factors, along with insulin and IGF-II. IGF-I stimulates proliferation and survival of various cell types including muscle, bone, and cartilage tissue. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/ autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH). Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR). Proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions, generates mature IGFs.

Entrez Symbol:

IGF1

Uniprot Name:

Insulin-like growth factor I

Uniprot ID:

P05019

Data PDF:

CRI500

Source:

E. coli

Molecular Weight:

Monomer, 7.7 kDa (70 aa)

Formulation:

Lyophilized from a sterile filtered solution containing 0.1% Trifluoroacetic acid (TFA).

Purity:

≥95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 1.5 ng/ml, determined by dose-dependent proliferation of MCF-7 cells.
Note: MCF-7 bioassay replaces the previous FDC-P1 bioassay.

Endotoxin Level:

< 0.1 EU/µg using USP <85>/ EP 2.6.14

Amino Acid Sequence:

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to reconstitute to a recommended concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for reconstitution.

Storage/Stability:

Upon receipt, store as supplied at -20°C to -80°C for up to one year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C, 3 months at -20° C to -80°C. For long term storage reconstitute in working aliquots containing 0.1% BSA and store at -80°C. Avoid repeated freeze-thaw cycle.