Fibroblast interferon, IFN-beta

Human

CSI12305A pdf (datasheet)

10 μg

$550.00 BUY

Human IFN-beta is a type I interferon, normally produced by fibroblasts, involved mainly in innate immune response, with antiviral and antiproliferative effects. It is considered first-line therapy for management of MS because of its immunomodulatory properties. It downregulates HLA class II molecules in antigen presenting cells. It also upregulates the expression of PDL-2 inhibitory molecules, which interact with their respective receptors on the T cells and induce apoptosis. It also inhibits proliferation of macrophages and so activation of autoreactive T cells.

The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17.

Interferon beta

P01574

CSI12305

E. coli

18.5 kDa (165 aa) monomer

Lyophilized from a 1 mg/ml solution containing 5% human albumin and 5% dextrose

> 98% by SDS-PAGE and RP-HPLC analysis

Viral resistance assay using human WISH cell line and VSV virus, or the monkey VERO cell line with EMCV virus

3.2 x 10⁷ IU/mg, determined in a viral resistance assay using human WISH cell line and VSV virus, or the monkey VERO cell line with EMCV virus

< 0.1 ng per μg of IFN-beta

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the protein to a concentration of 0.25 mg/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.

Store lyophilized protein at -20°C to -80°C for up to 1 year. Reconstituted protein is stable at -20°C to -80°C for longer storage. Avoid repeated freeze-thaw cycles.