Recombinant Human HB-EGF

Synonyms:

HBEGF, Diphtheria toxin receptor, DT-R

Species:

Human

Cat. No.:

CRH309B pdf (datasheet)

Quantity/Size:

100 µg

Price:

$395.00 BUY

Description:

Recombinant Human HB-EGF

Uniprot Name:

Proheparin-binding EGF-like growth factor

Uniprot ID:

Q99075

Data PDF:

CRH309

Source:

E. coli

Molecular Weight:

Monomer, 9.9 kDa (87 aa)

Formulation:

Lyophilized from a sterile-filtered solution containing 10 mM sodium phosphate, pH 7.5

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

Activity is determined by the ability to induce proliferation of 3T3 cells and is typically ≤ 0.2 ng/mL.

Specific Activity:

5 x 106 U/mg

Endotoxin Level:

≤1 EU/µg by kinetic LAL analysis

Amino Acid Sequence:

MDLQEADLDL LRVTLSSKPQ ALATPNKEEH GKRKKKGKGL GKKRDPCLRK YKDFCIHGEC KYVKELRAPS CICHPGYHGERCHGLSL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for reconstitution. A small amount of precipitate may be seen.

Storage/Stability:

Upon receipt, store desiccated at -20 °C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8 °C. For long term storage reconstitute in working aliquots containing 0.1% BSA and store at -80 °C. Avoid repeated freeze-thaw cycles.