Recombinant Human GPT / ALT, His Tag Active

Molecule:

GPT/ALT

Synonyms:

Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1

Species:

Human

Cat. No.:

CSI12679B pdf (datasheet)

Quantity/Size:

10 µg

Price:

$500.00 BUY

Description:

Alanine transaminase or ALT is found in serum and in various bodily tissues, but is most commonly associated with the liver. It catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and fatty liver disease.

Concentration:

0.5 mg/ml

Entrez Symbol:

GPT

Uniprot Name:

Alanine aminotransferase 1

Uniprot ID:

P24298

Data PDF:

CSI12679

Source:

E. coli

Molecular Weight:

56.8 kDa (516 aa, 1-496) homodimer

Tag:

His

Formulation:

Sterile filtered 20 mM Tris-HCl, pH 8.0, containing 2 mM DTT and 20% glycerol

Purity:

> 90% as determined by SDS-PAGE and RP-HPLC analysis.

Specific Activity:

> 100 U/mg, defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.

Amino Acid Sequence:

MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS

Storage/Stability:

Store at -80°C up to 1 year