Recombinant Human GM-CSF, GMP Grade

Synonyms:

Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF2, Molgramostin, Sargramostim

Species:

Human

Cat. No.:

CRG100B-GMP pdf (datasheet)

Quantity/Size:

100 μg

Price:

$3,365.00 BUY

Description:

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine, immune and inflammatory stimuli. GM-CSF is a growth factor for granulocyte-macrophage, erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF activates the effector functions of granulocytes, monocytes and eosinophils. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells.

This product is produced from E. coli and is manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. This product is manufactured, tested, and released in an ISO 9001:2015 certified facility and follows cGMP practices. USP chapter <1043> for ancillary materials considered in the manufacture of this product.

Entrez Symbol:

CSF2

Uniprot Name:

Granulocyte-macrophage colony-stimulating factor

Uniprot ID:

P04141

Data PDF:

CRG100-GMP

Source:

E. coli

Molecular Weight:

14.6 kDa (128 aa) monomer

Formulation:

Lyophilized (1mg/ml) sterile-filtered 10 mM sodium phosphate, pH 7.5

Purity:

> 98% by reducing and non-reducing SDS-PAGE

Biological Activity:

 ED50 < 0.2 ng/ml, determined by dose-dependent proliferation of human TF-1 cells.  

Specific Activity:

~1.6 x 104 IU/µg, which is calibrated against the recombinant Human GM-CSF WHO International Standard (NIBSC code 88/646).

Endotoxin Level:

< 0.02 EU/µg using USP <85> / EP 2.6.14

Amino Acid Sequence:

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Reconstitution:

Allow the vial and sterile water/Water for Injection to equilibrate to room temperature. Draw up desired volume for reconstitution with sterile syringe. Aseptically puncture through rubber stopper with sterile needle, inject the buffer to achieve the desired concentration (0.1-0.5 mg/mL). Swirl the vial gently, do not vortex. Allow protein to rehydrate for 10-15 minutes at room temperature with occasional gentle mixing.

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. 

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.