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Recombinant Human G-CSF / CSF3

Molecule:

CSF3

Synonyms:

CSF3OS, GCSF, granulocyte colony-stimulating factor, filgrastim, lenograstim, pluripoietin

Species:

Human

Cat. No.:

CRG300C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$4,160.00 / Special Price: $3,570.00 BUY

Description:

Recombinant Human GCSF

Entrez Symbol:

CSF3

Uniprot Name:

Granulocyte colony-stimulating factor

Uniprot ID:

P09919

Data PDF:

CRG300

Source:

E. coli

Molecular Weight:

18.8 kDa

Formulation:

Sterile-filtered aqueous solution containing 20 mM acetic acid, 50 mM NaCl.

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤60 pg/ml, determined by dose-dependent proliferation by NFS-60 cells.

Specific Activity:

> 1.7 x 107 U/mg
The specific activity of Human G-CSF is approximately 3.9 x 10^4 IU/μg, which is calibrated against recombinant human G-CSF WHO International Standard (NIBSC code: 09/136).

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilution should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.