Recombinant Human FLT3 Ligand, GMP Grade

Molecule:

FLT3L

Synonyms:

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine

Species:

Human

Cat. No.:

CRF110C-GMP pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$11,965.00 / Special Price: $10,290.00 BUY

Description:

Flt-3 ligand (Flt-3L) is a hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3. The predominant human Flt-3L isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. Flt-3L is widely expressed in various human and mouse tissues. Flt-3L has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.

This product is produced from E. coli and is manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. This product is manufactured, tested, and released in an ISO 9001:2015 certified facility and follows cGMP practices. USP chapter <1043> for ancillary materials considered in the manufacture of this product.

Entrez Symbol:

FLT3LG

Uniprot Name:

Fms-related tyrosine kinase 3 ligand

Uniprot ID:

P49771

Data PDF:

CRF110-GMP

Source:

E. coli

Molecular Weight:

17.7 kDa (155 aa) monomer

Formulation:

Lyophilized (1mg/ml) sterile-filtered 10 mM sodium phosphate, 50 mM NaCl, pH 7.5

Purity:

> 97% by reducing and non-reducing SDS-PAGE

Biological Activity:

 ED50 < 5 ng/ml, determined by dose-dependent proliferation of OCI-AML5 cells.  

Specific Activity:

~1.6 x 103 IU/µg, which is calibrated against the recombinant Human FLT-3 Ligand WHO International Standard (NIBSC code 96/532).

Endotoxin Level:

< 0.05 EU/µg using USP <85> / EP 2.6.14

Amino Acid Sequence:

MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT

Reconstitution:

Allow the vial and sterile water/Water for Injection to equilibrate to room temperature. Draw up desired volume for reconstitution with sterile syringe. Aseptically puncture through rubber stopper with sterile needle, inject the buffer to achieve the desired concentration (0.1-0.5 mg/mL). Swirl the vial gently, do not vortex. Allow protein to rehydrate for 10-15 minutes at room temperature with occasional gentle mixing.

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. 

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.