Recombinant Human Fibronectin Fragment GMP

Synonyms:

Fibronectin, FN

Species:

Human

Cat. No.:

CRI165C-GMP pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$450.00 BUY

Description:

Modified recombinant human fibronectin fragment can enhance retroviral-mediated gene transduction by aiding the co-localization of target cells and virions. Coated on the surface of cell culture dish, it significantly enhances the transfection ability of mammalian and insect cells; improves the efficiency of virus infection. The viral particle binds to the virus adhesion area (H-domain) of fibronectin, and target cells bind mainly through the interaction of cell surface integrin receptor VLA-5 and VLA-4 with the fibronectin C-domain and CS-1 site, respectively.

The manufacturing and testing of Recombinant Human Fibronectin Fragment GMP complies with ICH Q7 guidelines.

Entrez Symbol:

FN1

Uniprot Name:

Fibronectin

Uniprot ID:

P02751

Data PDF:

CRI165-GMP

Source:

E. coli

Molecular Weight:

62.2 kDa (574 aa) monomer

Formulation:

Lyophilized from sterile filtered 20 mM Tris-HCl, 150 mM NaCl, 5% Trehalose, 0.02% Tween-20, pH 8.5.

Purity:

> 95% by SDS-PAGE and HPLC analyses

Biological Activity:

Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture.  In this application, the recommended concentration for this effect is typically 1-5 mg/cm2.  Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50 μg/mL

Endotoxin Level:

< 0.1 EU/μg as determined by LAL method.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.