Description:
Recombinant Human FGF-21 *am
Uniprot Name:
Fibroblast growth factor 21
Molecular Weight:
19.5 kDa (182 aa) monomer
Formulation:
Lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.
Purity:
> 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 500 ng/ml, determined by proliferation of 3T3 cells with β-klotho and heparin
Specific Activity:
≥ 2.0 x 103 unit/mg
Endotoxin Level:
≤ 1 EUs/µg by kinetic LAL analysis.
Amino Acid Sequence:
MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
