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Recombinant Human FGF-basic / FGF-2 (154 aa)

Molecule:

FGF basic

Synonyms:

Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2

Species:

Human

Cat. No.:

CRH304C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$970.00 BUY

Description:

Recombinant Human FGF-2 Basic 154

Uniprot Name:

Fibroblast growth factor 2

Uniprot ID:

P09038

Data PDF:

CRH304

Source:

E. coli

Molecular Weight:

Monomer, 17.3 kDa (155 aa)

Formulation:

Lyophilized from sterile filtered solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5

Purity:

≥95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤5 ng/ml by dose-dependent 3T3 cell proliferation

Specific Activity:

≥ 2.0 x 105 U/mg

Endotoxin Level:

≤1 EU/µg

Amino Acid Sequence:

MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to reconstitute to a recommended concentration of 0.1 mg/mL and gently pipet solution up and down sides of vial. DO NOT VORTEX. Allow several minutes for reconstitution. A small amount of precipitate may be seen.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, store at 2-8°C for up to 1 month or prepare working aliquots and store at -20°C to -80°C for up to 3 months. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.