Synonyms:
FGF-9, Glia-Activating Factor, GAF, Heparin-binding growth factor-9, HBFG-9
Description:
Recombinant Human FGF-9
Uniprot Name:
Fibroblast growth factor 9
Molecular Weight:
23.4 kDa (207 aa) monomer
Formulation:
Lyophilized from a sterile filtered (0.2 µm) solution of 10 mM sodium phosphate, 25 mM sodium chloride, 50 mM sodium sulfate, pH 7.5
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 2.0 ng/ml, determined by 3T3 cell proliferation.
Specific Activity:
> 5.0 x 105 units/mg
Endotoxin Level:
≤ 1 EU/µg, determined by kinetic LAL
Amino Acid Sequence:
MPLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
