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Recombinant Human FGF-7, GMP Grade

Synonyms:

Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, HBGF-7, Keratinocyte growth factor

Species:

Human

Cat. No.:

CRF007B-GMP pdf (datasheet)

Quantity/Size:

100 μg

Price:

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Description:

Fibroblast growth factor 7 (FGF-7/KGF-1), is encoded by the FGF7 gene and binds to the b splice form of the tyrosine kinase receptor, FGFR2b. Affinity between FGF-7 and its receptor is increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51% amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 is important in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 is active on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species cross activity, human KGF-1 shares 96 % amino acid sequence identity with murine, and 92% with rat.
Manufacturing and testing of this product complies with ICH Q7 guidelines.

Entrez Symbol:

FGF7

Uniprot Name:

Fibroblast growth factor 7

Uniprot ID:

P21781

Data PDF:

CRF007-GMP

Source:

E. coli

Molecular Weight:

~18.9 kDa (163 aa) monomer

Formulation:

Lyophilized from sterile-filtered 20 mM phosphate buffer, 500 mM NaCl, 5% Trehalose, 0.02% Tween-20, pH 7.4

Purity:

> 98.0% by SDS-PAGE

Biological Activity:

ED50 < 10 ng/ml, determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells

Specific Activity:

> 1.0 × 10e5 U/mg

Endotoxin Level:

< 0.01 EU/μg protein as determined by LAL method

Amino Acid Sequence:

CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.