Synonyms:
FGF-5, Heparin-binding growth factor 5, HBGF-5, Smag-82
Description:
Recombinant Human FGF5
Uniprot Name:
Fibroblast growth factor 5
Molecular Weight:
Monomer, 27.7 kDa (252 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from sterile-filtered 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5
Purity:
≥ 95%, by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 10 ng/ml, determined by its ability to induce the proliferation of mouse 3T3 fibroblasts, in the presence of heparin
Specific Activity:
≥ 1.0 x 105 units/mg
Endotoxin Level:
≤ 1 EU/μg by kinetic LAL
Amino Acid Sequence:
MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG
Reconstitution:
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
