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Recombinant Human FGF-2 Basic, GMP Grade

Synonyms:

Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2r

Species:

Human

Cat. No.:

CRF001B-GMP pdf (datasheet)

Quantity/Size:

100 μg

Price:

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Description:

Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named “acidic fibroblast growth factor" (FGF1) and “basic fibroblast growth factor” (FGF2). Human FGF-acidic shares 54% amino acid sequence identity with FGF-basic. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion.
Manufacturing and testing of this product complies with ICH Q7 guidelines.

Entrez Symbol:

FGF2

Uniprot Name:

Fibroblast growth factor 2

Uniprot ID:

P09038

Data PDF:

CRF001-GMP

Source:

E. coli

Molecular Weight:

~16.5 kDa (147 aa) monomer

Formulation:

Lyophilized from sterile-filtered 20 mM Tris-HCl, 150 mM NaCl, 5% Trehalose, 0.02% Tween-20, pH 7.6

Purity:

> 98.0% by SDS-PAGE

Biological Activity:

ED50 < 0.05 ng/ml, determined by a cell proliferation assay using murine balb/c 3T3 cells

Specific Activity:

> 2.0 × 10e7 U/mg

Endotoxin Level:

< 0.01 EU/μg protein as determined by LAL method

Amino Acid Sequence:

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.