Epidermal Growth Factor, EGF, urogastrone

Human

CRE100B-GMP pdf (datasheet)

100 μg

$1,100.00 BUY

Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature human EGF shares 70% a.a. sequence identity with mature mouse and rat EGF.
Manufacturing and testing of this product complies with ICH Q7 guidelines.

EGF

Epidermal Growth Factor

P01133

CRE100-GMP

E. coli

6.2 kDa (53 aa)

Lyophilized from a sterile-filtered PBS, pH 7.4

> 98% by SDS-PAGE and HPLC analyses

ED50 < 0.1 ng/ml, by a dose-dependent cell proliferation assay using mouse Balb/c 3T3.

> 1.0 × 10e7 U/mg

< 0.01 EU/μg protein as determined by LAL method

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.