Recombinant Human EGF

Molecule:

EGF

Synonyms:

Beta-urogastrone, Urogastrone, EGF-URO, HMGF, PGF, URG

Species:

Human

Cat. No.:

CRE100C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$285.00 BUY

Description:

Recombinant Human EGF

Entrez Symbol:

EGF

Uniprot Name:

Epidermal growth factor

Uniprot ID:

P01133

Data PDF:

CRE100

Source:

E. coli

Molecular Weight:

6.2 kDa (53 aa)

Physical State:

Lyophilized

Formulation:

Sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 100 pg/ml, determined by a cell proliferation assay using mouse Balb/c 3T3.

Specific Activity:

> 1.0 × 107 U/mg The specific activity of Human EGF is approximately 1.3 x 10^3 IU/μg, which is calibrated against recombinant human EGF WHO International Standard (NIBSC code: 91/530).

Endotoxin Level:

< 1EU/μg determined by kinetic LAL analysis.

Amino Acid Sequence:

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of 0.1-1.0 mg/ml. The solution can then be further diluted in other aqueous buffers.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year