Synonyms:
Beta-urogastrone, Urogastrone, EGF-URO, HMGF, PGF, URG
Description:
Recombinant Human EGF
Uniprot Name:
Epidermal growth factor
Molecular Weight:
6.2 kDa (53 aa)
Physical State:
Lyophilized
Formulation:
Sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Purity:
> 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 < 100 pg/ml, determined by a cell proliferation assay using mouse Balb/c 3T3.
Specific Activity:
> 1.0 × 107 U/mg The specific activity of Human EGF is approximately 1.3 x 10^3 IU/μg, which is calibrated against recombinant human EGF WHO International Standard (NIBSC code: 91/530).
Endotoxin Level:
< 1EU/μg determined by kinetic LAL analysis.
Amino Acid Sequence:
NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of 0.1-1.0 mg/ml. The solution can then be further diluted in other aqueous buffers.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year
