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Recombinant Human CDK1 / Cyclin B1 Active (GST, His Tag)

Synonyms:

Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog & G2/mitoticspecific cyclin-B1, Cyclin B1, CCNB1

Species:

Human

Cat. No.:

CRC026A pdf (datasheet)

Quantity/Size:

10 μg

Price:

$585.00 BUY

Description:

Recombinant Human active protein kinase. Coexpression of human Cyclin-dependent kinase 1 (CDK1) amino acids M1- M297and human CCNB1, amino acids M1-V433, both N-terminally fused to GST-HIS6

Concentration:

Lot specific, < 1 mg/ml, determination by Bradford method using BSA as the standard

Uniprot Name:

Cyclin-dependent kinase 1, G2/mitotic-specific cyclin-B1

Uniprot ID:

P06493, P14635

Data PDF:

CRC026

Source:

Sf9 insect cells

Molecular Weight:

GST-CDK1: 63.882 kDa, predicted
GST-CCNB1: 78.925 kDa, predicted

Formulation:

50 mM HEPES, pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol

Biological Activity:

Assay conditions:
60 mM HEPES-NaOH, pH 7.5
3 mM MgCl2
3 mM MnCl2
3 µM Na-orthovanadate
1.2 mM DTT
2.5 µg / 50 µl PEG20.000
ATP (variable)
Substrate: Rb-CTF, 2.5 µg/ml
Recombinant CDK1/CycB1: 1 µg/ml
Filter binding assay
MSFC membrane (Millipore)

Specific Activity:

7.6 pmol/µg x min
ATP-Km : 0.8 µM

Storage/Stability:

Upon initial thaw, prepare working aliquots and store at -80°C. Avoid repeated freezethaw cycles.