Synonyms:
Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen
Description:
CD14 acts as a receptor for endotoxin (LPS) and is expressed on monocytes, macrophages, and neutrophils. CD14 is anchored to cells by glycosylphosphatidylinositol (GPI)-linkage and functions as a high affinity receptor of LPS-LBP-complexes. Together with TLR4 and a lymphocytic antigen (MD2) CD14 is the lipopolysaccharide (LPS)- Receptor and mediates innate immune response to bacterial lipoproteins. CD14 is present in a soluble form in human serum, urine and other body fluids which is directly secreted or derived from protease-dependent shedding of the membrane bound molecule. Soluble CD14 (sCD14) competes with membrane bound CD14 (mCD14) for LPS binding and is able to neutralize LPS-induced responses in vitro and in vivo and mediates the LPS-induced activation of non-CD14 expressing endothelial, epithelial and smooth muscle cells.
Concentration:
1 mg/ml prior to lyophilization.
Uniprot Name:
Monocyte differentiation antigen CD14
Formulation:
PBS, pH 7.2.
Purity:
90-95% by SDS-PAGE analysis.
Biological Activity:
Up to 10 µg/mL CD14 inhibits binding of FITC-LPS (0.5 µg/mL) to 6 x 105 CD14 CHO cell transfectants as determined by flow cytometry.
Endotoxin Level:
< 0.01 ng/mL by LAL analysis
Reconstitution:
Centrifuge vial prior to opening. Add 10 µL sterile distilled water to the vial. Further dilution can be made with PBS or other buffers.
Storage/Stability:
Store as supplied at -80°C for up to 1 year.
