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Recombinant Human MIP-5/CCL15

Molecule:

CCL15

Synonyms:

Macrophage inflammatory protein-5, HCC-2, Lkn-1

Species:

Human

Cat. No.:

CRM411B pdf (datasheet)

Quantity/Size:

25 μg

Price:

$275.00 BUY

Description:

Recombinant Human MIP-5/CCL15 is a single non-glycosylated polypeptide chain containing 92 amino acids.

Background: CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (a.a.) protein containing a putative signal peptide of 21 (a.a.) that is cleaved to generate a 92 a.a. residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% a.a. homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1gamma, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

Entrez Symbol:

CCL15

Uniprot Name:

C-C motif chemokine 15

Uniprot ID:

Q16663

Data PDF:

CRM411

Source:

E. coli

Molecular Weight:

~10.2 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4 + 100 mM NaCl.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.

Endotoxin Level:

Less than 1 EU/μg of rHuMIP-5/CCL15 as determined by LAL method.

Amino Acid Sequence:

QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.