Synonyms:
CAM2, CAMK2, CAMKB, caMK-II subunit beta, CaM-kinase II beta chain, CaM kinase II beta subunit, caM kinase II subunit beta, proline rich calmodulin-dependent protein kinase, calcium, calmodulin-dependent protein kinase type II beta chain
Description:
Product: Recombinant Human Full-Length protein, Histidine-tagged, expressed in insect cells. No special measures were taken to activate this kinase. Mass Spectrometry: The enzyme preparation was subjected to tryptic digestion followed by LCMS/MS. The resu
Concentration:
0.44 mg/ml (Total protein as measured using the Bradford protein assay with BSA as a standard).
Uniprot Name:
Calcium/calmodulin-dependent protein kinase type II subunit beta
Genbank Accession No:
NP_742078.1
Molecular Weight:
60.5 kDa
Physical State:
Frozen liquid
Formulation:
Sterile filtered liquid (Frozen) in 50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 +2 mM DTT + 50% Glycerol
Purity:
>90% by SDS-PAGE analysis.
Specific Activity:
774 nmol/min/mg (phosphate transferred to Autocamtide-2 peptide substrate (KKALRRQETVDAL) per minute per mg of total protein at 30�C. Activity determined at a final protein concentration of 1.67 �g/ml).
Amino Acid Sequence:
MSYYHHHHHHDYDIPTTENLYFQGITSLYKKAGTMATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHK
Storage/Stability:
Frozen liquid protein is stable at -80℃C for 6 months from date of purchase. For long term storage, aliquot and store at -80℃C. Avoid repeated freeze-thaw cycles.
Application notes:
The dilution buffer described is recommended for the radiometric assay format. For high throughput applications such as PolarScreen TM, Z'-LYTE TM, and LanthaScreen TM, modifications to this buffer are needed.
