Recombinant Human BDNF Animal Free

Molecule:

BDNF

Synonyms:

BDNF, Abrineurin, ANON2, BULN2

Species:

Human

Cat. No.:

CRB600D-AF pdf (datasheet)

Quantity/Size:

100 µg

Price:

$900.00 BUY

Description:

Brain Derived Neurotrophic Factor, or BDNF, is a nerve growth factor that supports neuron growth and survival. BDNF shares identical domains with two other neurotrophic factors known as, β-NGF and NT-3 (neurotrophin-3). BDNF binds with low affinity to a receptor known as LNGFR, which also binds NGF and NT-3, but mediates survival function by signaling through a high affinity receptor known as gp145/TrkB.

Human, mouse, rat and pig BDNF are all cross-reactive.

Made without animal-derived components in an animal-free facility.

Entrez Symbol:

BDNF

Uniprot Name:

Brain-derived neurotrophic factor

Uniprot ID:

P23560

Data PDF:

CRB600-AF

Source:

E. coli

Molecular Weight:

Homodimer (non-covalent), 13.6/27.3 kDa (120/240 aa)

Formulation:

Lyophilized from a sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 40 ng/ml, determined by the dose dependent proliferation of a neuroblastoma cell line stably expressing TrkB (BR6)

Endotoxin Level:

≤ 0.1 EU/µg by kinetic LAL

Amino Acid Sequence:

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.