B7-H4, B7H4, B7S1, B7X, B7h.5, PRO1291, VCTN1

Human

CRH028 pdf (datasheet)

100 μg

$499.00 BUY

B7-H4 is a B7 family member that negatively regulates T cell immunity by inhibiting of T cell proliferation, cytokine production, and cell cycle progression. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. B7-H4 ia an important negative regulator of innate immunity through growth inhibition of neutrophils. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance.

DNA-directed RNA polymerase

Q7Z8D3

CRH028

CHO cells

Lyophilized from sterile-filtered solution PBS.

≥98% by SDS-PAGE

Measured by the ablility to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well place coated with 500 ng/ml anti-CD3 and 10 μg/ml human B7H4:Fc. The presence of human B7H4:Fc at 10 μg/ml inhibited anti CD3 response by 30-50%.

<0.06 EU/μg purified protein as determined by LAL test

Reconstitute with 100 µL sterile water to yield 1 mg/mL solution. Add 1X PBS to the desired protein concentration.

Store unopened at -20°C to -80°C for at least 1 year. After reconstitution, prepare aliquots and store at -20°C to -80°C for up to 3 months .