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  • Recombinant Human Aspartate Aminotransferase Mitochondrial, Active

Recombinant Human Aspartate Aminotransferase Mitochondrial, Active

Synonyms:

Aspartate aminotransferase, mitochondrial, mAspAT, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2

Species:

Human

Cat. No.:

CRA029B pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$6,500.00 BUY

Description:

Two GOT isoenzymes are present in humans. They have high similarity. GOT1, the cytosolic isoenzyme, derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver. GOT2 is invloved in amino acid metabolism and the urea and tricarboxylic acid cycles. The 2 enzymes are homodimeric and demonstrate close homology.

Concentration:

0.5 mg/ml

Entrez Symbol:

GOT2

Uniprot Name:

Aspartate aminotransferase, mitochondrial

Uniprot ID:

P00505

EC number:

EC 2.6.1.1

Data PDF:

CRA029

Source:

E. coli

Molecular Weight:

47 kDa (424 aa) with a N-terminal 23 aa His tag

Formulation:

20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 10% glycerol and 1mM DTT.

Purity:

> 95% as determined by SDS-PAGE

Specific Activity:

> 60 U/mg protein
One unit is defined as the amount of enzyme that convert 1umole of α-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.

Amino Acid Sequence:

MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK

Storage/Stability:

Store at 2-8°C for immediate use within 2 weeks, or at -20°C to -80°C for up to 1 year. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.