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Recombinant Human Globular Adiponectin / gACRP30

Synonyms:

Adipocyte complement-related 30 kDa protein, ACRP30, Gelatin-binding protein, Adipocyte, C1q and collagen domain-containing protein

Species:

Human

Cat. No.:

CRA025C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,215.00 / Special Price: $2,750.00 BUY

Description:

Adiponectin is a 30 kDa multimeric protein and is secreted mainly by white adipose tissue, although other tissues express low levels of adiponectin too. Full-length human adiponectin comprises 244 amino acid residues, including a N-terminal hyper-variable region (amino acids from 1–18), followed by a collagen-like domain structurally homologous with collagen VIII and X, consisting of 22 Gly-XY repeats, and a C-terminal C1q-like globular domain (amino acids from 108–244). In contrast to humans, mouse adiponectin is a 247 amino acid long protein. Adiponectin is secreted from adipocytes into the bloodstream as three oligomeric complexes, including trimer (67 kDa), hexamer (140 kDa), and a HMW (300 kDa) multimer comprising of at least 18 monomers. The monomeric form of adiponectin is undetectable in native conditions. Globular adiponectin, the globular C1q domain of adiponectin generated from full-length protein by naturally occurring proteolysis is biologically active. gACRP30 is detected at a relatively high concentrations in the serum and is thought to play an important role in hyperglycemia, insulin resistance and cognitive decline in obesity. gACRP30 signals through receptors, AdipoR1 and AdipoR2. T-cadherin as a receptor for hexameric and HMW forms of adiponectin.

Entrez Symbol:

ADIPOQ

Uniprot ID:

Q15848

Data PDF:

CRA025

Source:

E. coli

Molecular Weight:

Monomer, 16.7 kDa (145 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 0.5 mM DTT, pH 7.5

Purity:

≥ 90% by reducing and non-reducing SDS-PAGE,

Biological Activity:

ED50 ≤ 2.0 µg/ml, determined by inhibition of M1 cell proliferation.

Specific Activity:

≥ 500 units/mg

Endotoxin Level:

≤ 1 EU/µg by kinetic LAL

Amino Acid Sequence:

MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.