Recombinant Mouse Adiponectin / ACRP30, FLAG-tag

Synonyms:

Adipocyte complement-related 30 kDa protein, ACRP30, Gelatin-binding protein, Adipocyte, C1q and collagen domain-containing protein

Species:

Mouse

Cat. No.:

CRA027B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$500.00 BUY

Description:

Adiponectin is a 30 kDa multimeric protein and is secreted mainly by white adipose tissue, although other tissues express low levels of adiponectin too. Full-length human adiponectin comprises 244 amino acid residues, including a N-terminal hyper-variable region (amino acids from 1–18), followed by a collagen-like domain structurally homologous with collagen VIII and X, consisting of 22 Gly-XY repeats, and a C-terminal C1q-like globular domain (amino acids from 108–244). In contrast to humans, mouse adiponectin is a 247 amino acid long protein. Adiponectin is secreted from adipocytes into the bloodstream as three oligomeric complexes, including trimer (67 kDa), hexamer (140 kDa), and a HMW (300 kDa) multimer comprising of at least 18 monomers. The monomeric form of adiponectin is undetectable in native conditions. Adiponectin is thought to play an important role in hyperglycemia, insulin resistance, cognitive decline in obesity, and signals through receptors, AdipoR1 and AdipoR2. T-cadherin as a receptor for hexameric and HMW forms of adiponectin.

Entrez Symbol:

Adipoq

Uniprot Name:

Adiponectin

Uniprot ID:

Q60994

Data PDF:

CRA027

Source:

HEK293 cells

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.4 µm filtered 50 mM phosphate buffer, 75 mM NaCl, pH 7.4

Purity:

> 98% as determined by HPLC and SDS-PAGE analyses

Biological Activity:

Full-length Recombinant Mouse Adiponectin activates AMP activated Protein Kinase in hepatocyte and activates AMPK in HepG2 Human hepatocytes at a concentration of 1µg/ml. Recombinant Mouse Adiponectin inhibits glucose production as shown by in-vitro gluconeogenesis assay in primary Rat hepatocytes.

Endotoxin Level:

< 0.1 ng/µg of ACRP30

Amino Acid Sequence:

EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.