Recombinant HIV-1 TAT Clade-B

Synonyms:

Protein Tat, Transactivating regulatory protein

Cat. No.:

CSI15819A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$440.00 BUY

Description:

Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication (Jones KA, 1994) and infectivity (Arya SK, 1985; Fisher AG, 1986). In addition, during acute infection, Tat is released extracellularly by infected cells (Chang HC, 1997; Ensoli B, 1990) and is taken up by neighboring cells where it transactivates viral replication (Ensoli B, 1993) and increases virus infectivity. HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.

Entrez Symbol:

tat

Uniprot Name:

Protein Tat

Uniprot ID:

P04608

Data PDF:

CSI15819

Source:

E. coli

Molecular Weight:

14 kDa (86 aa) monomer

Formulation:

Sterile-filtered and lyophilized with 0.1% glycerol.

Purity:

> 90.0% as determined by SDS-PAGE.

Biological Activity:

Immunoreactive with all sera of HIV-1 infected individuals.

Amino Acid Sequence:

MEPVDPRLEP WKHPGSQPKT ACTNCYCKKC CFHCQVCFIT KALGISYGRK KRRQRRRPPQ GSQTHQVSLS KQPTSQSRGD PTGPKE.

Reconstitution:

It is recommended to reconstitute the lyophilized HIV-1 TAT in sterile deionized water at > 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage/Stability:

Store at -20°C to -80°C

Application notes:

Recognized by anti-Tat (HIV-1) polyclonal antibody.
Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.