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Recombinant Bovine/Porcine FGF-2

Molecule:

FGF2/FGF-basic

Synonyms:

Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor

Species:

Porcine

Cat. No.:

CRF023B pdf (datasheet)

Quantity/Size:

50 μg

Price:

$500.00 BUY

Description:

Fibroblast Growth Factor-basic (bFGF) plays a significant role in the process of wound healing, limb and nervous system development, tumor growth and is a potent inducer of angiogenesis. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. Bovine and porcine FGF2 share a high degree of sequence homology with similar activity for both species.

Entrez Symbol:

FGF2

Uniprot Name:

Fibroblast growth factor 2

Uniprot ID:

P03969 (bovine)

Data PDF:

CRF023x

Source:

E. coli

Molecular Weight:

17.25 kDa (155 aa) monomer

Physical State:

Lyophilized

Formulation:

PBS containing 1% HSA

Purity:

> 97% by SDS-PAGE and HPLC analyses

Biological Activity:

ED50 < 0.1 ng/ml, by cell proliferation assay using quiescent NR6R-3T3 fibroblasts

Specific Activity:

3 x 10e6 units/mg

Endotoxin Level:

< 1 EU/µg by LAL analysis

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.