Specificity

Recognizes both native and recombinant rat TNF-alpha

Range

82 pg/ml - 20,000 pg/ml

Sensitivity

< 25 pg/ml

Incubation

4 hours, 45 min   or

1.5 hours 45 min with overnight

Sample Types

Serum
Plasma
Cell culture supernatant

Cross Reaction

No cross-reactivity with any of the cytokines tested (e.g., rat CINC2, CINC-3, CNTF, Fractalkine, IL-1α, IL- 1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP- α, β- NGF, TIMP-1, VEGF).

Kit Contents

Pre-coated 12 strip plate, biotinylated secondary antibody, standards, controls, buffers, Streptavidin-HRP, TMB, Stop Reagent.