Rat Anti-Mouse VEGFR-1 (Clone 5F13) mAb

Synonyms:

Vascular endothelial growth factor receptor 1, VEGFR-1, Embryonic receptor kinase 2, Fms-like tyrosine kinase 1, FLT-1, Tyrosine-protein kinase receptor FLT

Species:

Mouse

Cat. No.:

CMV801 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$770.00 BUY

Description:

Vascular Endothelial Growth Factor (VEGF or VEGF-A) family members are major mediators of vasculogenesis and angiogenesis. Specifically, biological activities attributed to VEGFs include: mitogenic activity on endothelial cells, increased permeability of endothelial cells to proteins, stimulation of monocyte migration across endothelial cells and angiogenic activity. Three VEGF family receptors have been described: Flt-1 (fms-like tyrosine kinase) also known as VEGF R1, KDR (kinase-insert domain-containing receptor) also known as Flk-1 and VEGF R2, and Flt-4 also known as VEGF R3. The three receptors contain seven extracellular immunoglobulin-like domains and share substantial sequence homology. In addition, neuropilin-1, a neuronal receptor, also acts as a co-receptor for VEGF when expressed on vascular endothelial cells, endothelial cell progenitors and monocytes. VEGF R1 is expressed primarily on endothelial cells but is also found on human peripheral blood monocytes. Through its endothelial mitogenic and hyperpermeability activities, VEGF influences a variety of immune functions related to wound healing and blood protein traffic across endothelial barriers.

Uniprot Name:

Vascular endothelial growth factor receptor 1

Uniprot ID:

P35969

Data PDF:

CMV801

Specificity:

Recognizes mouse VEGFR-1. No reactivity to mouse VEGFR-2 and VEGFR3.

Host Species:

Rat

Immunogen:

Recombinant mouse VEGFR-1 extracellular domain

Isotype:

IgG2

Clone:

5F13

Formulation:

Lyophilized from sterile filtered PBS

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial briefly prior to opening. Add 500 µl sterile PBS to the vial for a concentration of 200 µg/ml. Gently mix, allow a few minutes to solubilize completely. Do not vortex.

Application notes:

Western blot: suggested dilution 1:500-1000
IHC-P: suggested dilution 1:50-100

Storage/Stability:

Lyophilized antibody is stable for at least 1 year at -20°C to -80°C. Reconstituted antibody is stable for six months in working aliquots at -20°C to-80°C. Avoid repeated freeze-thaw cycles.